SNLS
Seed DNA/RNA Extraction Mini Kit
The Seed DNA/RNA Extraction Mini Kit (magnetic bead based) enables fast and efficient purification of high-quality DNA and RNA from seeds and fruit tissues. Using superparamagnetic beads, the kit selectively binds nucleic acids while removing polysaccharides, polyphenols, and other plant-derived inhibitors. It supports sample inputs of around 100–150 mg and produces pure nucleic acids ideal for PCR, qPCR, sequencing, and molecular breeding applications. The protocol is compatible with manual or automated processing for high reproducibility and ease of use.
Method
Magnetic Bead
Research Use Only
Sample Material
Whole seeds, dehulled seeds, ground seed powder, germinated seeds
200 μL
20 - 40min
30 - 60 μL
> 10Kbp
∼ 20-30 μg
∼ 1.8 - 2.0
PCR, RT-PCR
Restriction digestion
Ligation and transformation
Sequencing
Screening
Quantification
Other downstream processes
Room temperature (18 – 25 °C)
SKUs (Pack Size)
50 Preps, 100 Preps, 250 Preps