SNLS
Plasmid DNA Extraction Mini Kit
The Plasmid DNA Extraction Mini Kit provides a rapid and reliable method to isolate high-purity plasmid DNA from bacterial cultures such as E. coli. Utilizing silica membrane spin column technology combined with a streamlined alkaline lysis procedure, the kit efficiently purifies plasmid DNA free from genomic DNA, RNA, proteins, and other contaminants. The purified plasmid DNA is suitable for a wide range of molecular biology applications including cloning, sequencing, PCR, transfection, and in vitro transcription.
Method
Spin Column
Certification
Research Use Only
Research Use Only
Sample Material
Plasmid DNA, bacterial cultures, recombinant clones, transformed cells
Sample Amount
50 - 200 μL
50 - 200 μL
Processing time
20 - 30 min
20 - 30 min
Elution volume
50 - 100 μL
50 - 100 μL
Fragment size
> 10Kbp
> 10Kbp
Theoretical binding capacity
12 μg
12 μg
Purity at A260/A280
∼ 1.8 - 2.0
∼ 1.8 - 2.0
Applications
PCR, RT-PCR
Restriction digestion
Ligation and transformation
Sequencing
Screening
Quantification
Other downstream processes
Storage Temperature
Room temperature (18 – 25 °C)
Room temperature (18 – 25 °C)
SKUs (Pack Size)
50 Preps, 100 Preps, 250 Preps