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SNLS

Stool/Fecal - DNA/RNA Extraction Mini Kit


The Stool/Fecal DNA/RNA Extraction Mini Kit provides a reliable and efficient method for isolating high-quality genomic DNA and total RNA from fresh or frozen stool and fecal samples. Designed to overcome challenges posed by complex biological matrices and PCR inhibitors such as humic acids and bile salts present in stool, the kit utilizes mechanical disruption via bead beating and optimized chemical lysis buffers combined with silica membrane spin columns or magnetic bead technology. This results in nucleic acids of superior purity and yield, suitable for comprehensive downstream applications including microbial community analysis, infectious disease diagnostics, metagenomics, and host genetic studies.

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a close up of a blue and purple structure

Meth​od

Spin Column
Certification
Research Use Only
 

Sample Material

Stool, fecal samples, rectal swabs, animal droppings, environmental fecal material


Sample Amount ​
200 μL
 
Processing time
20 - 40min
 
Elution volume
30 - 60 μL
 
Fragment size
> 10Kbp
 
Theoretical binding capacity
20-30 μg
 
Purity at A260/A280
∼ 1.8 - 2.0
 
Applications ​

PCR, RT-PCR

Restriction digestion

Ligation and transformation

Sequencing

Screening

Quantification  

Other downstream processes

Storage Temperature
Room temperature (18 – 25 °C)


SKUs (Pack Size)

50 Preps, 100 Preps, 250 Preps


DNA Catcher Brouchers


RNA Catcher Brouchers